Mouse monoclonal antibody to SARS-CoV2 (ACE2 binding domain) (Clone:2G1)

Catalogue Number: 34-1134-ABO

Manufacturer:Abeomics
Type:Monoclonal Primary Antibody - Unconjugated
Shipping Condition:Blue Ice
Unit(s): 100 ul, 50 ul
Host name: Mouse
Clone: 2G1
Isotype: IgG1
Immunogen: Recombinant SARS-CoV2 S-Protein ACE2 binding domain expressed in and purified from E. coli.
Application: ICC, IF, WB

Description

Description: Immunofluorescent analysis of transfected HEK293 cells with the SARS-CoV2-bd-construct under high magnification, stained with mouse mAb to SARS-CoV2-bd, dilution 1:1,000, in green. Cells were costained with rabbit pAb to HSP60, RPCA-HSP60, dilution 1:2,000, in red. The blue is Hoechst staining of nuclear DNA. This antibody reveals overexpression of SARS-CoV2-bd protein presumanbly in lysosomes only in transfected cells, while the HSP60 antibody labels mitochondria in all cells. This antibody was raised against a recombinant construct of the SARS-CoV-2 spike or S-protein which includes the entire region which interacts with ACE2. The specific binding to ACE2 is essential for viral internalization and infection. We designed this construct based on amino acids 308-541 in the S-protein sequence in Isolate Wuhan-Hu-1, complete genome. This is a defined globular domain recently shown to include all of the amino acids necessary for ACE2 binding. The construct was expressed in and purified from E. coli and includes an N-terminal His-tag and other vector derived sequence shown underlined below. Amino acids which interact directly with the ACE2 protein are printed in bold.

Additional Text

Gene Name

S

Antibody Clonality

Monoclonal

Application Notes

WB: 1:1,000-1:2,000. ICC/IF: 1:1,000

Gene ID

43740568

Uniprot ID

P0DTC2

Storage Note

Shipped on ice. Store at 4°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.