Mouse monoclonal antibody to SARS-CoV2 (ACE2 binding domain) (Clone:2G1)
Catalogue Number: 34-1134-ABO
34-1134 Image
34-1134 Image
34-1134 Image
Fig 1:-Cells were transfected with the DNA encoding the S-protein segment in PROT-SARS-CoV2-bd which was inserted in the pCI-Neo-Mod expression vector (5). This antibody produces clean and specific staining of transfected cells which stain identically with RPCA-SARS-CoV2-bd, rabbit polyclonal antibody to the same immunogen. The nuclei of transfected and untransfected cells are shown in blue with DAPI DNA stain.
34-1134 Image
Fig 2:-Cells were transfected with DNA encoding the S-protein ACE2 binding site in PROT-SARS-CoV2-bd which was inserted into pCI-Neo-Mod or pCI-Neo-GFP eukaryotic expression vectors, which express either the insert only or the insert fused with GFP (5). Lane 1 shows a crude homogenate of untransfected control cells, lane 2 shows a homogenate of cells expressing SARS-CoV2-bd and lane 3 shows a homogenate of cells expressing GFP-SARS-CoV2-bd fusion. This antibody was used at a dilution of 1:3,000 and produces clean and specific staining of bands of the expected size as shown in green. The blot was also stained with rabbit polyclonal control antibody to HSP60, RPCA-HSP60, at a dilution of 1:5,000 in red.
| Manufacturer: | Abeomics |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 50 ul |
| Host name: | Mouse |
| Clone: | 2G1 |
| Isotype: | IgG1 |
| Immunogen: | Recombinant SARS-CoV2 S-Protein ACE2 binding domain expressed in and purified from E. coli. |
| Application: | ICC, IF, WB |
Description
Description: Immunofluorescent analysis of transfected HEK293 cells with the SARS-CoV2-bd-construct under high magnification, stained with mouse mAb to SARS-CoV2-bd, dilution 1:1,000, in green. Cells were costained with rabbit pAb to HSP60, RPCA-HSP60, dilution 1:2,000, in red. The blue is Hoechst staining of nuclear DNA. This antibody reveals overexpression of SARS-CoV2-bd protein presumanbly in lysosomes only in transfected cells, while the HSP60 antibody labels mitochondria in all cells. This antibody was raised against a recombinant construct of the SARS-CoV-2 spike or S-protein which includes the entire region which interacts with ACE2. The specific binding to ACE2 is essential for viral internalization and infection. We designed this construct based on amino acids 308-541 in the S-protein sequence in Isolate Wuhan-Hu-1, complete genome. This is a defined globular domain recently shown to include all of the amino acids necessary for ACE2 binding. The construct was expressed in and purified from E. coli and includes an N-terminal His-tag and other vector derived sequence shown underlined below. Amino acids which interact directly with the ACE2 protein are printed in bold.
Additional Text
Gene Name
S
Antibody Clonality
Monoclonal
Application Notes
WB: 1:1,000-1:2,000. ICC/IF: 1:1,000
Gene ID
43740568
Uniprot ID
P0DTC2
Storage Note
Shipped on ice. Store at 4°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
34-1134 Datasheet
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