Mouse monoclonal antibody to SARS-CoV2 (ACE2 binding domain) (Clone:5G8)
Catalogue Number: 34-1135-ABO
34-1135 Image
34-1135 Image
34-1135 Image
Fig 1: Immunofluorescent analysis of HEK293 cells transfected with pCI-Neo-Mod vector (5) including SARS-CoV2-bd cDNA and stained with mouse mAb to SARS-CoV2-bd, with dilution 1:1,000, in red. The blue is Hoechst staining of nuclear DNA. This antibody reveals expression of SARS-CoV2-bd protein only in transfected cells. DAPI reveals the nuclear DNA of both transfected and non-transfected cells.
34-1135 Image
Fig 2 :-Western blot analysis of HEK293 cell lysates using mouse mAb to SARS-CoV2-bd protein, with dilution 1:3,000 in green: [1] protein standard, [2] non-transfected cells, [3] cells transfected with pCI-Neo-Mod containing the SARS-CoV2-bd cDNA, and [4] cells transfected with pCI-Neo-GFP vector expression construct containing containing the SARS-CoV2-bd cDNA. The band at 25kDa mark in the transfected cells demonstrates expression of SARS-CoV-bd protein, and the band at about 50kDa corresponds to a GFP-SARS-CoV2-bd fusion protein. The same blot was simultaneously probed with rabbit pAb to HSP60, RPCA-HSP60, dilution 1:5,000, in red, revealing a single band at 60kDa in both transfected and non-transfected cells.
| Manufacturer: | Abeomics |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 50 ul |
| Host name: | Mouse |
| Clone: | 5G8 |
| Isotype: | IgG1 |
| Immunogen: | Recombinant SARS-CoV2 S-Protein ACE2 binding domain expressed in and purified from E. coli. |
| Application: | ICC, IF, WB, IHC |
Description
Description: This antibody was raised against a recombinant construct of the SARS-CoV2 spike or S-protein which includes the entire region which interacts with ACE2. The specific binding to ACE2 is essential for viral internalization and infection. We designed this construct based on amino acids 308-541 in the S-protein sequence in Isolate Wuhan-Hu-1, complete genome. This is a defined globular domain recently shown to include all of the amino acids necessary for ACE2 binding. The construct was expressed in and purified from E. coli and includes an N-terminal His-tag and other vector derived sequence shown underlined below. Amino acids which interact directly with the ACE2 protein are printed in bold.
Additional Text
Gene Name
S
Antibody Clonality
Monoclonal
Application Notes
WB: 1:1,000-1:2,000. ICC/IF: 1:3,000-5,000
Gene ID
43740568
Uniprot ID
P0DTC2
Storage Note
Shipped on ice. Store at 4°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
34-1135 Datasheet
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