Polyclonal Antibody to NF-kB-p65(Phospho-Ser276)

Catalogue Number: 35-1010-ABO

Manufacturer:	Abeomics
Type:	Polyclonal Primary Antibody - Unconjugated
Alias:	p65\|\|NF-kB3
Shipping Condition:	Blue Ice
Unit(s):	100 ug, 50 ug
Host name:	Rabbit
Clone:
Isotype:	IgG
Immunogen:	Peptide sequence around phosphorylation site of serine 276(R-P-S(p)-D-R) derived from Human NF-kB-p65.
Application:	IF, WB, IHC

Overview
Datasheets

Description

Description: NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NF-kB1/p105, NF-kB1/p50, REL and NF-kB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1.

Additional Text

Gene Name

RELA

Gene ID

5970

Uniprot ID

Q04206

Antibody Clonality

Polyclonal

Application Notes

Western blotting: 1:500-1:1000

Storage Note

Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

35-1010 Datasheet

35-1010 MSDS

Price

£449.00

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