Polyclonal Antibody to IKK Alpha (Phospho-Thr23)
Catalogue Number: 35-1125-ABO
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35-1125 Image
35-1125 Image
35-1125 Image
Figure 1: Western blot analysis of extracts from 293 cells untreated or treated with TNF using IKK a(Phospho-Thr23) Antibody 35-1125 .
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Figure 2: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using IKK a(Phospho-Thr23) Antibody 35-1125 (left) or the same antibody preincubated with blocking peptide(right).
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Figure 3: Western blot analysis of extracts from Hela cells, treated with EGF or calf intestinal phosphatase (CIP), using IKK a (Phospho-Thr23) Antibody 35-1125 .
| Manufacturer: | Abeomics |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | I kappa-B kinase alpha|| I-kappa-B kinase 1|| IKK-A|| IKK-alpha|| IKK1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug, 50 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Peptide sequence around phosphorylation site of threonine 23 (L-G-T(p)-G-G) derived from Human IKK a. |
| Application: | WB, IHC |
Description
Description: Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NF-kB2/p100 associated with RelB, inducing its proteolytic processing to NF-kB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3. Phosphorylates 'Ser-10' of histone H3 at NF-kappa-B-regulated promoters during inflammatory responses triggered by cytokines. Yuan ZQ, et al.(2002) J Biol Chem; 277(33): 29973-82. Ozes ON, et al. (1999) Nature; 401(6748): 82-5.
Additional Text
Gene Name
CHUK
Gene ID
1147
Uniprot ID
O15111
Antibody Clonality
Polyclonal
Application Notes
Predicted MW: 85kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Storage Note
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
35-1125 Datasheet
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