Polyclonal Antibody to IkB-beta (Phospho-Ser23)
Catalogue Number: 35-1274-ABO
35-1274 Image
35-1274 Image
35-1274 Image
Figure 1: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IkB-b(Phospho-Ser23) Antibody 35-1274 (left) or the same antibody preincubated with blocking peptide(right).
35-1274 Image
Figure 2: Western blot analysis of extracts from HT29 cells, treated with CA or calf intestinal phosphatase (CIP), using IkB-b (Phospho-Ser23) Antibody 35-1274 .
| Manufacturer: | Abeomics |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | I-kappa-B-beta, IKB-B, IKBB, NF-kappa-BIB, NF-kappaB inhibitor beta |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug, 50 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Peptide sequence around phosphorylation site of serine 23 (L-G-S(p)-L-G) derived from Human IkB-b. |
| Application: | WB, IHC |
Description
Description: Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further IKBA-dependent inactivation. Association with inhibitor kappa B-interacting NKIRAS1 and NKIRAS2 prevent its phosphorylation rendering it more resistant to degradation, explaining its slower degradation. Shirane, M. et al. (1999) J Biol Chem 274, 28169-28174. DiDonato J, et al. (1996) Mol Cell Biol 16(4): 1295-304
Additional Text
Gene ID
4793
Gene Name
NFKBIB
Uniprot ID
Q15653
Antibody Clonality
Polyclonal
Application Notes
Predicted MW: 48kd, Immunohistochemistry: 1:50~1:100
Storage Note
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
35-1274 Datasheet
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