Polyclonal Antibody to Histone H3 (Tri-Methyl-Lys27)
Catalogue Number: 35-1347-ABO
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35-1347 Image
35-1347 Image
35-1347 Image
Figure 1: Western blot analysis of extracts from HT29 and C6 cells using Histone H3 (Tri-Methyl-Lys27) Antibody 35-1347 .
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Figure 2: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Histone H3 (Tri-Methyl-Lys27) Antibody 35-1347 .
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Figure 3: Immunofluorescence staining of methanol-fixed MEF cells using Histone H3 (Tri-Methyl-Lys27) Antibody 35-1347 .
| Manufacturer: | Abeomics |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | H3/a||H3/c||H3/d||H3/f||H3/h |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug, 50 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Peptide sequence around Tri-Methylation site of lysine 27(A-R-K(tri-methyl)-S-A) derived from Human Histone H3. |
| Application: | IF, WB, IHC |
Description
Description: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Additional Text
Uniprot ID
P68431
Gene ID
8350; 8351; 8352; 8353; 8354; 8355; 8356; 8357; 8358; 8968
Antibody Clonality
Polyclonal
Gene Name
H3C1, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8
Application Notes
Predicted MW: 17kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Storage Note
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
35-1347 Datasheet
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