Polyclonal Antibody to NF-kB-p65 (Ab-254)

Catalogue Number: 35-1367-ABO

Manufacturer:Abeomics
Type:Polyclonal Primary Antibody - Unconjugated
Alias:NF-kB3|| RELA|| TF65|| Transcription factor p65|| p65
Shipping Condition:Blue Ice
Unit(s): 100 ug, 50 ug
Host name: Rabbit
Clone:
Isotype: IgG
Immunogen: Peptide sequence around aa.252~256 (F-R-T-P-P) derived from Human NF-kB-p65.
Application: IHC

Description

Description: NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NF-kB1/p105, NF-kB1/p50, REL and NF-kB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex

Additional Text

Gene Name

RELA

Gene ID

5970

Uniprot ID

Q04206

Antibody Clonality

Polyclonal

Application Notes

Predicted MW: 65kd, Immunohistochemistry: 1:50~1:100

Storage Note

Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.