Polyclonal Antibody to NF-kB-p65 (Ab-276)
Catalogue Number: 35-1368-ABO
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35-1368 Image
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Figure 1: Western blot analysis of extracts from Hela cells using NF-kB-p65(Ab-276) Antibody 35-1368 and the same antibody preincubated with blocking peptide.
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Figure 2: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NF-kB-p65(Ab-276) Antibody 35-1368 (left) or the same antibody preincubated with blocking peptide(right).
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Figure 3: Immunofluorescence staining of methanol-fixed Hela cells using NF-kB-p65(Ab-276) Antibody 35-1368 .
| Manufacturer: | Abeomics |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | NF-kB3|| RELA|| TF65|| Transcription factor p65|| p65 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug, 50 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Peptide sequence around aa.274~278 (R-P-S-D-R) derived from Human NF-kB-p65. |
| Application: | IF, WB, IHC |
Description
Description: NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NF-kB1/p105, NF-kB1/p50, REL and NF-kB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex
Additional Text
Gene Name
RELA
Gene ID
5970
Uniprot ID
Q04206
Antibody Clonality
Polyclonal
Application Notes
Predicted MW: 65kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Storage Note
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
35-1368 Datasheet
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