Figure 1: Western blot analysis of extracts from HT29 cells using NF-kB-p105/p50(Ab-337) Antibody 35-1374 and the same antibody preincubated with blocking peptide.
35-1374 Image
Figure 2: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NF-kB-p105/p50(Ab-337) Antibody 35-1374 (left) or the same antibody preincubated with blocking peptide(right).
Description: NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NF-kB1/p105, NF-kB1/p50, REL and NF-kB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively.
Additional Text
Antibody Clonality
Polyclonal
Application Notes
Predicted MW: 50 120 kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Storage Note
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.