Figure 1: Western blot analysis of extracts from 293 and 3T3 cells using IkB-a (Ab-32/36) Antibody 35-1471 .
35-1471 Image
Figure 2: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IkB-a (Ab-32/36) Antibody 35-1471 (left) or the same antibody preincubated with blocking peptide(right).
35-1471 Image
Figure 3: Immunofluorescence staining of methanol-fixed Hela cells using IkB-a (Ab-32/36) Antibody 35-1471 .
Description: Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to tranlocate to the nucleus and activate transcription.
Additional Text
Gene ID
4792
Gene Name
NFKBIA
Uniprot ID
P25963
Antibody Clonality
Polyclonal
Application Notes
Predicted MW: 39kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Storage Note
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.