Polyclonal Antibody to Histone H2A.X (Ab-139)
Catalogue Number: 35-1593-ABO
35-1593 Image
35-1593 Image
35-1593 Image
35-1593 Image
Figure 1: Western blot analysis of extracts from HepG2 and 293 cells using Histone H2A.X(Ab-139) Antibody 35-1593 .
35-1593 Image
Figure 2: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Histone H2A.X(Ab-139) Antibody 35-1593 (left) or the same antibody preincubated with blocking peptide(right).
35-1593 Image
Figure 3: Immunofluorescence staining of methanol-fixed Hela cells using Histone H2A.X(Ab-139) Antibody 35-1593 .
| Manufacturer: | Abeomics |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | H2A.X|| H2AFX|| H2a/x|| HIST5-2AX |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug, 50 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Peptide sequence around aa.137~141 (Q-A-S-Q-E) derived from Human Histone H2A.X. |
| Application: | IF, WB, IHC |
Description
Description: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation
Additional Text
Gene ID
3014
Uniprot ID
P16104
Gene Name
H2ax
Antibody Clonality
Polyclonal
Application Notes
Predicted MW: 15kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Storage Note
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
35-1593 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations