Screen Quest™ No Wash Potassium Channel Assay Kit
Catalogue Number: 36550-AAT
| Manufacturer: | AAT Bioquest |
| Type: | Detection Kit |
| Shipping Condition: | RT |
| Unit(s): | 1 Plate |
Description
Description: Potassium (K+) ion channel plays an important role in regulating fundamental biological processes including heart rate, hormone and neurotransmitter secretion, water and electrolyte balance. Potassium ion channel has been considered as drug targets for disease indications including arrhythmia, pain, diabetes, neurological dysfunctions etc. The permeability of Tl+ through K+ channel has been widely used to assay K+ channel. The cells that express K+ channel of interests (e.g. hERG, Kv1.3, Kir2.1, KATP) are pre-loaded with a Tl+ sensitive dye. The dye is non-fluorescent and is permeable to cell membrane. Once inside the cell, the non-fluorescent AM ester dye is cleaved by endogenous esterase into a negatively charges dye that stays inside cells. When a stimulus buffer containing low dose of Tl+ is added to cells, the Tl+ flows across the K+ channel and binds to Tl+ sensitive dye, generating a fluorescent signal. This signal is proportional to the activity of K+ channel. If an antagonist or antagonist is added to the cells, the fluorescent signal decreases or increases respectively, to reflect the inhibited or stimulated activity of K+ channel.
Additional Text
Storage Note
Multiple;|6 months
36550 Protocol
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations