Rabbit Polyclonal Antibody to DYKDDDDK-tag
Catalogue Number: 45-1004-ABO
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45-1004 Image
45-1004 Image
45-1004 Image
45-1004 Image
Figure-1 : Western blot analysis of DYKDDDDK-tag Antibody at 1 µg/ml on DYKDDDDK-tagged fusion protein (100, 50 & 10 ng).
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Figure-2 : Western blot analysis of DYKDDDDK-tag Antibody at 1 µg/ml on DYKDDDDK-tagged fusion protein (40ng).
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Figure-3 : Western blot analysis of DYKDDDDK-tag Antibody at 1 µg/ml on C-terminal & N-terminal of DYKDDDDK tagged fusion proteins (10-100ng)expressed in E. coli cell lysate.
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Figure-4 : Immunofluorescence analysis of DYKDDDDK-tag Antibody at 10 µg/ml on HEK293 cells transfected with Flag-tagged CD4 protein.
| Manufacturer: | Abeomics |
| Type: | Tag antibodies |
| Shipping Condition: | Blue Ice |
| Unit(s): | 40 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | A synthetic peptide DYKDDDDK conjugated to KLH |
| Application: | ELISA |
Description
Description: The DYKDDDDK peptide is a small component of the epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. DYKDDDDK-tag Antibody, pAb, Rabbit can be used to detect DYKDDDDK-tagged proteins.
Additional Text
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Storage Note
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Application Notes
ELISA: 0.01-0.05 µg/ml Western blot: 1-2 µg/ml Immunofluorescence:10-20 µg/ml
45-1004 Datasheet
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