Mouse Monoclonal Antibody to cGFP-tag (Clone : 5E5G11)
Catalogue Number: 45-1008-ABO
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45-1008 Image
45-1008 Image
45-1008 Image
45-1008 Image
Figure-1 : Western blot analysis of cGFP-tag Antibody (Clone: 5E5G11) at 1 µg/ml on GFP fusion protein, (1-3: 100, 25, & 10 ng respectively, 4: Negative control).
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Figure-4 : Western blot analysis of cGFP-tag Antibody (Clone: 5E5G11) on a different variants of GFP protein such as eGFP, cGFP and AcGFP1. IRDye 800 Conjugated Goat Anti-mouse IgG was used as secondary antibody.
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Figure-2 : Western blot analysis of cGFP-tag Antibody (Clone: 5E5G11) on GFPuv protein, in 1: OFP transfecting 293 cell lysate, 2: EGFP transfecting 293 cell lysate, 3: GFPuv protein (5 ng), 4: 293 cell lysate (as negative control).
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Figure-3 : Western blot analysis of cGFP-tag Antibody (Clone: 5E5G11) at 1 µg/ml on GFP fusion protein, (1-3: 200, 40 & 8 ng respectively, 4: Negative control).
| Manufacturer: | Abeomics |
| Type: | Tag antibodies |
| Shipping Condition: | Blue Ice |
| Unit(s): | 40 ug |
| Host name: | Mouse |
| Clone: | 5E5G11 |
| Isotype: | IgG2b |
| Immunogen: | Purified recombinant cGFP fusion protein |
| Application: | ELISA |
Description
Description: The coral green fluorescent protein (cGFP) from vector pRNATin-H1.2/Neo is a versatile marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression. Mouse Anti-cGFP-tag Monoclonal Antibody can be used to detect cGFP fusion protein in Western blot analyses.
Additional Text
Purification
Protein G purified
Antibody Clonality
Monoclonal
Storage Note
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Application Notes
ELISA: 0.1-1.0 µg/ml Western blot: 1.0-2.0 µg/ml Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 1 mg/ml.
45-1008 Datasheet
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