Rabbit Polyclonal Antibody to CBP-tag
Catalogue Number: 45-1015-ABO
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45-1015 Image
45-1015 Image
45-1015 Image
Figure-1 : Western blot analysis of CBP-tag Antibody at 1 µg/ml on CBP tagged fusion protein (200 & 40 ng) expressed in E. coli Cell Lysate.
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Figure-2 : Western blot analysis of CBP-tag Antibody at 1 µg/ml on CBP tagged fusion protein (50, 20, 10 & 5 ng)expressed in E. coli cell lysate. IRDy 800 Conjugated Goat Anti-Rabbit IgG was used as Secondary Antibody.
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Figure-3 : Western blot analysis of CBP-tag Antibody on CBP-tagged protein, Lane-1: CBP-tagged protein from the transfected CHO cell lysates (as input control), Lane-2: Immuno-precipitates of CBP-tagged protein from the transfected CHO cell lysates with CBP Tag Antibody, 3: Immuno-precipitates of the CBP-tagged protein from the transfected CHO cell lysates with Rabbit IgG Control (Whole Molecule).
| Manufacturer: | Abeomics |
| Type: | Tag antibodies |
| Shipping Condition: | Blue Ice |
| Unit(s): | 40 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | A synthetic CBP tag peptide KRRWKKNFIAVSAANRFKKISSSGAL conjugated to KLH |
| Application: | ELISA, WB |
Description
Description: CBP tag is a calmodulin-binding peptide, KRRWKKNFIAVSAANRFKKISSSGAL, derived from skeletal/cardiac muscle myosin light chain kinase 2. Rabbit Anti-CBP-tag Polyclonal Antibody is developed in rabbit hosts using a synthetic CBP tag peptide conjugated to KLH. CBP-tag Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography. The antibody is suitable for detecting CBP fusion proteins.
Additional Text
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Application Notes
ELISA: 0.05-0.2 µg/mlWestern blot: 1-2 µg/ml Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Storage Note
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
45-1015 Datasheet
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