Rabbit Polyclonal Antibody to Avi-tag

Catalogue Number: 45-1018-ABO

Manufacturer:Abeomics
Type:Tag antibodies
Shipping Condition:Blue Ice
Unit(s): 40 ug
Host name: Rabbit
Clone:
Isotype: IgG
Immunogen: Avi tag peptide conjugated to KLH
Application: ELISA, WB

Description

Description: Well-characterized antibodies for epitope tags have been widely used in the study of protein expression in various systems. The Avi tag is a biotin-acceptor peptide1, GLNDIFEAQKIEWHE. The 15-residue peptide served as a substrate mimic for biotin ligase (BirA), which usually recognizes the much larger protein domain. Rabbit Anti-Avi-tag Polyclonal Antibody is developed in rabbit using a synthetic Avi tag peptide conjugated to KLH.

Additional Text

Purification

Affinity Purified

Antibody Clonality

Polyclonal

Application Notes

ELISA: 0.05-0.2 µg/mlWestern blot: 1-2 µg/ml Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.

Storage Note

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.