Rabbit Polyclonal Antibody to Avi-tag
Catalogue Number: 45-1018-ABO
45-1018 Image
45-1018 Image
45-1018 Image
Figure-1 : Western blot analysis of Avi-tag Antibody at 1 µg/ml on Avi-tagged fusion protein (1000, 200, 40 & 8 ng)expressed in E. coli cell lysate.
45-1018 Image
Figure-2 : Western blot analysis of Avi-tag Antibody at 1 µg/ml on N-terminal & C-terminal of Avi tagged fusion protein (100, 50, 20 & 10 ng) expressed in E. coli cell lysate, IRDye 800 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody.
| Manufacturer: | Abeomics |
| Type: | Tag antibodies |
| Shipping Condition: | Blue Ice |
| Unit(s): | 40 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Avi tag peptide conjugated to KLH |
| Application: | ELISA, WB |
Description
Description: Well-characterized antibodies for epitope tags have been widely used in the study of protein expression in various systems. The Avi tag is a biotin-acceptor peptide1, GLNDIFEAQKIEWHE. The 15-residue peptide served as a substrate mimic for biotin ligase (BirA), which usually recognizes the much larger protein domain. Rabbit Anti-Avi-tag Polyclonal Antibody is developed in rabbit using a synthetic Avi tag peptide conjugated to KLH.
Additional Text
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Application Notes
ELISA: 0.05-0.2 µg/mlWestern blot: 1-2 µg/ml Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Storage Note
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
45-1018 Datasheet
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