Rabbit Polyclonal Antibody to RFP-tag
Catalogue Number: 45-1022-ABO
45-1022 Image
45-1022 Image
45-1022 Image
45-1022 Image
Figure-1 : Western blot analysis of RFP-tag Antibody at 1 µg/ml on RFP-tagged fusion protein (200 & 40 ng) expressed in E. coli cell lysate.
45-1022 Image
Figure-2 : Western blot analysis of RFP-tag Antibody at 1 µg/ml on RFP-tagged fusion protein (40 ng, 8 ng, 1.6 ng, 0.8 ng ) expressed in E. coli cell lysate.
45-1022 Image
Figure-3 : Western blot analysis of RFP-tag Antibody at 1 µg/ml on red fluorescent protein variants (mCherry, DsRed, DeRed2, mOrange, tdTomato), IRDye 800 Conjugated Goat Anti-Rabbit IgG was used as Secondary Antibody.
| Manufacturer: | Abeomics |
| Type: | Tag antibodies |
| Shipping Condition: | Blue Ice |
| Unit(s): | 40 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | RFP epitope tag peptide VNGHEFEIEGEGEGR conjugated to KLH |
| Application: | ELISA |
Description
Description: Well-characterizated antibodies against short-sequence epitope tags are common in the study of protein expression in systems. RFP-tags are composed of 15-residue peptides, VNGHEFEIEGEGEGR, derived from amino acids 22-36 of red fluorescent protein (RFP) from the Discosoma sea anemone.
Additional Text
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Storage Note
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Application Notes
ELISA: 0.05-0.2 µg/ml Western blot: 1-2 µg/ml . Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
45-1022 Datasheet
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