Rabbit Polyclonal Antibody to GFP
Catalogue Number: 45-1042-ABO
45-1042 Image
45-1042 Image
45-1042 Image
45-1042 Image
45-1042 Image
Figure-1 : Western blot analysis of GFP Antibody at 1 µg/ml on GFP fusion protein (200, 100, 50 & 10 ng), IRDye 800 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody.
45-1042 Image
Figure-4 : Western blot analysis of GFP Antibody on immunoprecipitates from lysate expressing GFP, 1: Input control material for lysate expressing GFP protein, 2: Negative control with isotype control, 3: Immunprecipitation with GFP Antibody.
45-1042 Image
Figure-2 : Western blot analysis of GFP Antibody at 1 µg/ml on GFP uv fusion protein (100, 40, 20 & 10 ng), IRDye 800 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody.
45-1042 Image
Figure-3 : Western blot analysis of GFP Antibody at 1 µg/ml on YFP fusion protein, IRDye 800 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody.
| Manufacturer: | Abeomics |
| Type: | Tag antibodies |
| Shipping Condition: | Blue Ice |
| Unit(s): | 40 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Purified recombinant full-length GFP protein |
| Application: | ELISA, WB |
Description
Description: Green fluorescence protein (GFP) is a 27 kDa protein derived from jellyfish Aequorea victoria. It emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP has become a very useful tool as a fusion protein to report gene expression, trace cell lineage and define subcellular protein localizations. YFP differs from GFP due to a mutation at T203Y. The antibodies raised against full-length GFP should also detect YFP and other variants. Rabbit Anti-GFP Polyclonal Antibody is developed in rabbit using purified recombinant full-length GFP protein. This polyclonal antibody is highly purified from rabbit antiserum by immunoaffinity chromatography.
Additional Text
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Storage Note
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Application Notes
ELISA: 0.05-0.2 µg/ml.Western blot: 1-2 µg/ml
45-1042 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations