Mouse Monoclonal Antibody to CBP Tag (Clone : 1D11E5)

Catalogue Number: 45-1069-ABO

Manufacturer:Abeomics
Type:Tag antibodies
Shipping Condition:Blue Ice
Unit(s): 40 ug
Host name: Mouse
Clone: 1D11E5
Isotype: IgG2b, kappa
Immunogen: CBP tag peptide conjugated to KLH
Application: ELISA

Description

Description: CBP affinity tag is a useful tag in protein study which can be added to the N or C terminus of proteins of interest through DNA recombinant technology. The tag is derived from muscle myosin light-chain kinase. The tag comprises 26 amino acid residues with the molecular weight of 4 kDa and sequence of KRRWKKNFIAVSAANRFKKISSSGAL. CBP tag has the relatively high affinity for calmodulin (CaM). CBP-tagged proteins can be purified from crude cell extracts through CaM affinity resin. CBP Tag antibody is a useful tool in analysis of CBP-tagged proteins. CBP Tag Antibody, mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes harvested from mouse immunized with CBP tag peptide conjugated to KLH.

Additional Text

Purification

Protein A purified

Antibody Clonality

Monoclonal

Storage Note

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Application Notes

Western blot: 1-2 µg/ml. Immunoprecipitation: 1-2 µg/ml. ELISA: 0.001-0.002 µg/ml.