Biotinylated Mouse Monoclonal Antibody to Pembrolizumab (Clone : 10E12C6)
Catalogue Number: 45-1084-ABO
45-1084 Image
45-1084 Image
45-1084 Image
Figure-1 : ELISA binding of Pembrolizumab antibody (Clone: 10E12C6) binds with Pembrolizumab, Coating antigen: Pembrolizumab at 1 µg/ml, Pembrolizumab antibody dilution start from 1,000 ng/ml, EC50= 5.77 ng/ml.
45-1084 Image
Figure-2 : ELISA blocking of Pembrolizumab antibody (Clone: 10E12C6) with Pembrolizumab binding with Human PD-1 recombinant protein, Coating antigen: Pembrolizumab at 1 µg/ml, PD-1-Fc-biotin final concentration: 20 ng/ml, Anti-Pembrolizumab antibody dilution start from 30 µg/ml,IC50= 0.37 µg/ml
| Manufacturer: | Abeomics |
| Type: | Monoclonal Primary Antibody - Conjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 40 ug |
| Host name: | Mouse |
| Clone: | 10E12C6 |
| Isotype: | IgG1, kappa |
| Immunogen: | Pembrolizumab |
| Application: | ELISA |
Description
Description: Pembrolizumab (Keytruda) is a humanized monoclonal antibody that is approved by the U.S. Food and Drug Administration for the treatment of patients with unresectable or metastatic melanoma. Pembrolizumab binds to the human cell surface receptor PD-1 (Programmed Cell Death Protein 1) and blocks its interaction with PD-L1, resulting in the activation of T-cell-mediated immune responses against tumor cells. Anti-Pembrolizumab Antibody (10E12C6)[BIOTIN], mAb, Mouse is produced from a hybridoma resulting from the fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with Pembrolizumab.
Additional Text
Purification
Protein A purified
Antibody Clonality
Monoclonal
Storage Note
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Application Notes
ELISA detection: 0.01-0.1 µg/ml ELISA blocking: 10-50 µg/ml
45-1084 Datasheet
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