Rabbit Monoclonal Antibody to His Tag (C-term) (Clone : 25B6E11)

Catalogue Number: 45-1089-ABO

Manufacturer:Abeomics
Type:Tag antibodies
Shipping Condition:Blue Ice
Unit(s): 40 ug
Host name: Rabbit
Clone: 25B6E11
Isotype: IgG
Immunogen: 6x His synthetic peptide coupled to KLH
Application: ELISA, FACS, ICC, IF, IP, WB

Description

Description: The rabbit immune system generates antibody diversity and optimizes affinity. technology to generate high affinity and specific monoclonal rabbit antibodies. Anti-His Tag (C-term) Antibody (25B6E11), mAb, Rabbit is specific to six histidine tags placed at C-terminal and internal regions of fusion proteins. This antibody can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells.

Additional Text

Purification

Protein A purified

Antibody Clonality

Monoclonal

Storage Note

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Application Notes

ELISA: 0.05-0.2 µg/ml Western Blot: 1-2 µg/ml Immunoprecipitation (IP): 2 µg/ml Immunocytochemistry/Immunofluorescence (ICC/IF): 1 µg/ml Flow Cytometry: 1 µg/ml