Rabbit Monoclonal Antibody to DYKDDDDK Tag (Clone : 114F12C8)
Catalogue Number: 45-1095-ABO
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45-1095 Image
45-1095 Image
45-1095 Image
Figure-1 : ELISA binding of DYKDDDDK Tag Antibody (Clone: 114F12C8)on DYKDDDDK Tagged protein, Coating antigen: DYKDDDDK-tagged fusion protein at 1 µg/ml, DYKDDDDK Tag Antibody dilution start from 1,000 ng/ml, EC50= 47.78 ng/ml.
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Figure-2 : Western blot analysis of DYKDDDDK Tag Antibody (Clone: 114F12C8) at 0.2 µg/ml on N-terminal of DYKDDDDK-tagged fusion protein (1-4: 25 ng, 10 ng, 5 ng & 1 ng respectively), C-terminal of DYKDDDDK-tagged fusion protein (5-8: 25 ng, 10 ng, 5 ng, 1 ng), Internal positions DYKDDDDK-tagged fusion protein (9-13: 50 ng, 25 ng, 10 ng, 5 ng, 1 ng respectively), IRDye 800 conjugated Goat anti-Rabbit IgG (H&L) was used as secondary antibody at 0.125 µg/ml.
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Figure-3 : Western blot analysis of DYKDDDDK Tag Antibody (Clone: 114F12C8) on immunoprecipitation from cell lysates containing DYKDDDDK fusion protein of 1: DYKDDDDK antibody (4 µg) + Hela cell lysate containing N-terminal DYKDDDDK tagged fusion protein, 2: Rabbit IgG (4 µg)+ Hela cell lysate containing N-terminal DYKDDDDK tagged fusion protein, 3: N-terminal DYKDDDDK tagged fusion protein (Input), 4: DYKDDDDK antibody (4 µg) + Hela cell lysate containing C-terminal DYKDDDDK tagged fusion protein, 5: Rabbit IgG (4 µg)+ Hela cell lysate containing C-terminal DYKDDDDK tagged fusion protein, 6: C-terminal DYKDDDDK tagged fusion protein (Input) at 0.2 µg/ml, IRDye800 conjugated Goat anti-Rabbit IgG (H&L) was used as secondary antibody at 0.125 µg/ml.
| Manufacturer: | Abeomics |
| Type: | Tag antibodies |
| Shipping Condition: | Blue Ice |
| Unit(s): | 40 ug |
| Host name: | Rabbit |
| Clone: | 114F12C8 |
| Isotype: | IgG |
| Immunogen: | DYKDDDDK synthetic peptide coupled to KLH |
| Application: | ELISA |
Description
Description: The rabbit immune system generates antibody diversity and optimizes affinity. technology to generate the high affinity and specificity monoclonal rabbit antibodies. DYKDDDDK Tag Antibody, mAb, Rabbit specific to DYKDDDDK tags placed at C-terminal, N-terminal and internal regions of fusion proteins. The antibody can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify DYKDDDDK fusion proteins in bacteria and mammalian cells.
Additional Text
Purification
Protein A purified
Antibody Clonality
Monoclonal
Storage Note
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Application Notes
ELISA: 0.05-0.2 µg/ml Western Blot: 0.2-1 µg/ml
45-1095 Datasheet
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