HRP conjugated Rabbit Monoclonal Antibody to DYKDDDDK Tag (Clone : 114F12C8)
Catalogue Number: 45-1096-ABO
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45-1096 Image
45-1096 Image
45-1096 Image
Figure-1 : ELISA binding of HRP conjugated DYKDDDDK Tag Antibody (Clone: 114F12C8)with DYKDDDDK Tagged fusion protein, Coating antigen: DYKDDDDK-tagged fusion protein at 1 µg/ml, HRP conjugated DYKDDDDK Tag Antibody dilution start from 1,000 ng/ml, EC50= 20.21 ng/ml.
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Figure-2 : Western blot analysis of HRP conjugated DYKDDDDK Tag Antibody (Clone: 114F12C8) at 1 µg/ml on C-terminal of DYKDDDDK-tagged fusion protein (1-4: 25 ng, 10 ng, 5 ng, 1 ng respectively).
45-1096 Image
Figure-3 : Western blot analysis of HRP conjugated DYKDDDDK Tag Antibody (Clone: 114F12C8) at 1 µg/ml on 1-4: N-terminal DYKDDDDK-tagged fusion protein (25 ng, 10 ng, 5 ng, 1 ng).
| Manufacturer: | Abeomics |
| Type: | Tag antibodies |
| Shipping Condition: | Blue Ice |
| Unit(s): | 40 ug |
| Host name: | Rabbit |
| Clone: | 114F12C8 |
| Isotype: | IgG |
| Immunogen: | DYKDDDDK synthetic peptide coupled to KLH |
| Application: | ELISA |
Description
Description: The rabbit immune system generates antibody diversity and optimizes affinity. technology to generate the high affinity and specificity monoclonal rabbit antibodies. DYKDDDDK Tag Antibody [HRP], mAb, Rabbit specific to DYKDDDDK tags placed at C-terminal, N-terminal and internal regions of fusion proteins. The antibody can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify DYKDDDDK fusion proteins in bacteria and mammalian cells.
Additional Text
Purification
Protein A purified
Antibody Clonality
Monoclonal
Application Notes
ELISA: 0.05-0.2 µg/ml Western blot: 1-2 µg/ml
Storage Note
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
45-1096 Datasheet
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