GLP-2 EIA "RUO"
Catalogue Number: 48-GP2HU-E01.1-ALP
| Manufacturer: | Alpco Diagnostics |
| Type: | Human ELISA Kit |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 96 Wells |
| Host name: | |
| Clone: | |
| Isotype: | |
| Immunogen: | |
| Range: | 0.412 - 100 ng/mL |
| Sensitivity: | 0.412 ng/mL |
| Sample type: | Plasma, Serum |
| Sample size: | 25 µL |
| Application: | IA, EIA |
Description
Description: The GLP-2 ELISA is based on a competitive enzyme immunoassay using a combination of highly specific antibody to human GLP-2 and the biotin-avidin affinity system. Standards, samples, biotinylated human GLP-2, and rabbit anti-GLP-2 antibody are added to the microplate wells coated in goat anti-rabbit IgG for the competitive immunoreaction. After incubation and plate washing, horse radish peroxidase (HRP)-labeled streptavidin (SA) is added to form an HRP-labeled SA - biotinylated GLP-2 - antibody complex on the surface of the wells. Finally, HRP enzyme activity is determined by o-phenylenediamine dihydrochloride (OPD) and the concentration of human GLP-2 is calculated. The proglucagon gene is expressed in both pancreatic A cells and intestinal L cells. Tissue-specific posttranslational processing of proglucagon by prohormone convertase produces different proglucagon derived peptides (PGDPs) in both the pancreas and intestine. The most notable pancreatic PGDP is glucagon, whereas intestinal L cells produce several structurally related peptides, including glucagon-like peptide 1 (GLP-1) and 2 (GLP-2), as well as glicentin and oxyntomodulin which contain the glucagon sequence in their molecules. Among PGDPs, GLP-2 has been found to result in intestinal epithelial proliferation.
Additional Text
Regulatory Statuses
Research Use Only. Not for Use in Diagnostic Procedures.
Short Description
The GLP-2 ELISA is designed for determination of GLP-2 in Plasma and Serum samples. For Research Use Only. Analytical sensitivity 0.412 ng/mL Dynamic range 0.412-100 ng/mL
Storage Note
store at 2-8C
48-GP2HU-E01.1 Protocol
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