StarGate® Acceptor Vector pASG-IBA145

Catalogue Number: 5-4145-001-IBA

Manufacturer:	IBA GmbH
Shelf Life:	12 Months
Type:	Vector
Shipping Condition:	RT
Unit(s):	5 ug

Overview
Datasheets

Description

Description: The pASG-IBA145 vector is designed for expression of recombinant proteins with an N-terminal Twin-Strep-tag® and C-terminal His-tag in E. coli. The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells, F1 and ColE1 origin for a high plasmid copy number, and the inducible tetracycline promoter/operator for the regulated expression of proteins. The vector can be combined with any E. coli strain since the tet-promoter works independently of the genetic background of E. coli. Expression of the recombinant protein is induced by addition of anhydrotetracycline, which is a derivative of tetracycline that does not exhibit antibiotic activity. Please note that cloning into pASG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pASG-IBA vectors with Esp3I, another option via a so-called Entry Vector is possible.

Additional Text

Short Description

Cytoplasmic E. coli expression vector with N-terminal Twin-Strep-tag® and C-terminal His-tag

5-4145-001 Datasheet

5-4145-001 Sequence

5-4145-001 MSDS

Price

£266.00

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