StarGate® Acceptor Vector pESG-IBA145

Catalogue Number: 5-4545-001-IBA

Manufacturer:	IBA GmbH
Shelf Life:	12 Months
Type:	Vector
Shipping Condition:	RT
Unit(s):	5 ug

Overview
Datasheets

Description

Description: The pESG-145 vector is designed for high-level, stable, and non-replicative transient expression of target proteins with an N-terminal Twin-Strep-tag® and C-terminal His-tag in mammalian cells. The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells as well as the F1 and ColE1 origin for a high plasmid copy number. In addition, it carries the human cytomegalovirus (CMV) immediate-early promoter for high-level expression in a wide range of mammalian cells, the neomycin resistance gene for selection of stable cell lines, and the SV40 ori for episomal replication in cell lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. HEK293T, COS-1, COS-7). Please note that cloning into pESG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pESG-IBA vectors with Esp3I, another option via a so-called Entry Vector is possible.

Additional Text

Short Description

Mammalian expression vector with N-terminal Twin-Strep-tag® and C-terminal His-tag

5-4545-001 Datasheet

5-4545-001 Sequence

5-4545-001 MSDS

Price

£266.00

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