StarGate® Acceptor Vector pDSG-IBA45

Catalogue Number: 5-5214-001-IBA

Manufacturer:	IBA GmbH
Shelf Life:	12 Months
Type:	Vector
Shipping Condition:	RT
Storage Condition:	2-8°C
Unit(s):	5 ug

Overview
Datasheets

Description

Description: pDSG-IBA45 is a small transient expression vector encoding an N-terminal Strep-tag®II as well as a C-terminal His-tag and was especially developed for the use in combination with the MEXi mammalian expression system. The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells and the ColE1 replication origin (pUC) for a high plasmid copy number. In addition, it contains the human cytomegalovirus (CMV) immediate-early promoter for high-level expression and the origin of replication from Epstein-Barr Virus (oriP) for extrachromosomal replication driven by EBNA-1 expressed by MEXi-293E cells. Please note that cloning into pDSG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pDSG-IBA vectors with Esp3I, another option via a so-called Entry Vector is possible.

Additional Text

Short Description

Mammalian expression vector with N-terminal Strep-tag®II and C-terminal His-tag

5-5214-001 Datasheet

5-5214-001 Sequence

5-5214-001 MSDS

Price

£266.00

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