ReadiLink™ KLH Conjugation Kit
Catalogue Number: 5502-AAT
| Manufacturer: | AAT Bioquest |
| Type: | Conjugation Kit |
| Shipping Condition: | RT |
| Unit(s): | 2 reactions |
Description
Description: Keyhole Limpet Hemocyanin (KLH) is one of the most commonly used carriers in the conjugation of peptides for antibody production. Mariculture keyhole limpet hemocyanin (mcKLH) is a hemocyanin from the Concholepas concholepas mollusk with immunogenic properties similar to KLH but is more stable and efficient as a carrier protein for the production of antibodies to haptens and peptides. It contains numerous sites per molecule for effective conjugation of peptides and other antigens using amine-reactive or carboxyl-reactive crosslinkers. mcKLH is currently the industry standard for antibody production against a hapten or peptide. This ReadiLink™ KLH Conjugation kit is primarily optimized for the simple preparation of hapten-carrier conjugates for immunization and antibody production. The ReadiLink™ KLH Conjugation kit is one-step conjugation of a hapten to a carrier protein using the carboxyl-reactive carbodiimide as the crosslinker. The resulting conjugate is used for eliciting an immune response and antibody production against the hapten. The carboxyl-reactive carbodiimide reacts with exposed carboxyl and amino groups on peptides and proteins to form stable bonds. These kits contain mcKLH formulated in buffers compatible with the carboxyl-reactive carbodiimide reactions and desalt spin columns, which offer exceptional protein recovery by simple centrifugation step.
Additional Text
Storage Note
Multiple;|6 months
5502 Protocol
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