CD8 Fab-TACS® Agarose column Starter Kit, mouse

Catalogue Number: 6-3305-002-IBA

Manufacturer:	IBA GmbH
Type:	Cell Separation Products
Shipping Condition:	RT
Storage Condition:	2-8°C
Unit(s):	1 kit

Overview

Description

Description: Fab-TACS® - Traceless Affinity Cell Selection - is a positive cell isolation approach based on our Strep-tag® technology. CD8 Fab-TACS® Agarose Column Starter Kit allows label-free isolation of murine cytotoxic CD8+ T cells from splenocytes or other cell suspensions. CD8 is a co-receptor that associates with the T cell receptor and interacts with MHC class I molecules. Strep-Tactin® TACS Agarose columns are filled with Strep-Tactin® coated agarose macrobeads developed for cell isolation. Low affinity Fab fragments fused to a Twin-Strep-tag® (Fab-Streps) bind to the matrix. Target cells adhere to the matrix based on the exclusive binding of the Fab-Strep to a surface marker. Non-target cells are efficiently washed away. In a final step, the addition of biotin causes the elution of the target cells and the Fab-Streps. After elution, the Fab-Streps self-dissociate from the cell surfaces due to their low affinity. The label-free authentic target cells are now ready for further downstream applications.

Additional Text

Short Description

Label-free positive isolation of CD8+ T cells from murine splenocytes

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