Strep-Tactin® TACS Agarose 50% suspension
Catalogue Number: 6-6350-010-IBA
| Manufacturer: | IBA GmbH |
| Type: | Cell Separation Products |
| Shipping Condition: | RT |
| Storage Condition: | 2-8°C |
| Unit(s): | 20 ml |
Description
Description: Strep-Tactin® TACS Agarose contains agarose macrobeads coated with the streptavidin variant Strep-Tactin®. A special crosslinking permits the use for cell isolation and depletion approaches. It was developed for column-based Traceless Affinty Cell Selection (Fab-TACS®/Nano-TACS®). In this approach, Fab fragments fused to a Twin-Strep-tag® (Fab-Streps) or nanobodies (Nano-Streps) are bound to Strep-Tactin® on the agarose matrix. After specifically capturing cells, Fab-/Nano-Streps are released from the Strep-Tactin® Agarose by biotin addition. The eluted cells are ready for further downstream applications. The same principle is applicable for exosome isolation (Fab-TACS® for exosomes). Alternatively to Fab-Streps/Nano-Streps, it is possible to bind Twin-Strep-tagged, desthiobiotinylated or biotinylated antibodies (irreversible binding) to this resin. This makes Strep-Tactin® TACS Agarose also suitable for negative cell selection or depletion approaches. The suspension can be used for packing of gravity flow columns since the resin is optimized for column affinity chromatography. To pack a column with 1 ml resin bed volume, 2 ml 50% suspension are necessary.
Additional Text
Short Description
50% suspension for traceless affinity cell and exosome isolation
SUPPORT
outstanding technical support
PRODUCT
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DELIVERY
we offer free delivery to UK universities and non profit organisations