6-HEX phosphoramidite [5'-Hexachlorofluorescein phosphoramidite]
Catalogue Number: 6026-AAT
| Manufacturer: | AAT Bioquest |
| Shelf Life: | 6 months |
| Type: | Fluorescent Reagents |
| Shipping Condition: | RT |
| Unit(s): | 100 umoles |
Description
Description: There are several ways of labeling an oligonucleotide with fluorescein. The choice of label is diversified further, depending on the spectral requirements. 5’-fluorescein-CE phosphoramidite, derived from the single isomer 6-carboxyfluorescein, 5’-hexachlorofluorescein-CE phosphoramidite (HEX) and 5’-tetrachlorofluorescein-CE phosphoramidite (TET) can all be used to efficiently label an oligonucleotide at the 5’-end. Labeling the 3’-end of an oligo with fluorescein can be achieved using 3’-Fluorescein CPGs. Standard cleavage and deprotection with ammonium hydroxide liberates the fluorescein-labeled oligo when using any of these supports.
Additional Text
Storage Note
Freeze (< -15 °C); Minimize light exposure;|6 months
6026 Protocol
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