Catalogue Number: 703-606-155-JIR
Manufacturer: | Jackson ImmunoResearch |
Shelf Life: | 12 months |
Preservative: | 0.05% Sodium azide |
Physical state: | Freeze-dried solid |
Type: | Polyclonal Secondary Antibody - Conjugated |
Shipping Condition: | RT |
Unit(s): | 0.3 mg |
Host name: | Donkey |
Clone: | |
Isotype: | |
Immunogen: | IgG (H+L) |
Description: F(ab')2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region. F(ab')2 fragments have two antigen-binding Fab portions linked together by disulfide bonds and therefore they are divalent. The average molecular weight is about 110 kDa. They are used for specific applications, such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G. Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule chicken IgY. It also reacts with the light chains of other chicken immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, goat, guinea pig, syrian hamster, horse, human, mouse, rabbit, rat and sheep serum proteins, but it may cross-react with immunoglobulins from other species.
AffiniPure
Polyclonal
Donkey Anti-Chicken IgG (H+L) secondary antibody, conjugated to Alexa Fluor® 647. This is an affinity purified F(ab′)2 Fragment polyclonal antibody and has been tested and/or adsorbed to ensure minimal cross reactivity with Bovine, Goat, Guinea Pig, Syrian Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep serum proteins.
1:100 - 1:800 for most applications Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
Store freeze-dried solid at 2-8°C.|One year from date of rehydration.