AffiniPure™ Donkey Anti-Human IgM, Fc5µ Fragment Specific (min X Bov,Hrs Sr Prot)

Catalogue Number: 709-005-073-JIR

Manufacturer:	Jackson ImmunoResearch
Shelf Life:	12 months
Preservative:	No preservative
Physical state:	Liquid
Type:	Polyclonal Secondary Antibody - Unconjugated
Shipping Condition:	RT
Storage Condition:	2-8°C
Unit(s):	1.5 mg
Host name:	Donkey
Clone:
Isotype:
Immunogen:	IgM, Fc5µ fragment

Overview
Datasheets

Description

Description: Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the Fc5µ portion of the human IgM heavy chain but not with human IgG, IgA, or the light chains of human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine and horse serum proteins, but it may cross-react with IgM from other species.

Additional Text

Purification

AffiniPure

Antibody Clonality

Polyclonal

Short Description

Donkey Anti-Human IgM, Fc5μ fragment unconjugated secondary antibody. This is an affinity purified whole IgG polyclonal antibody and has been tested and/or adsorbed to ensure minimal cross reactivity with Bovine and Horse serum proteins.

Application Notes

10-20 µg / ml Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.