Catalogue Number: 90-2081-ABO
Manufacturer: | Abeomics |
Type: | Human ELISA Kit |
Shipping Condition: | Blue Ice |
Unit(s): | 1 x 96 Tests |
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Application: | ELISA |
Description: This assay employs an antibody specific for anti-human IL-1Alphacoated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1Alphapresent in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-humanIL-1Alphaantibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1Alphabound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This immunoassay kit allows for the in vitro quantitative determination of Human IL-1 Alpha concentrations in serum, plasma and other biological fluids. Detection Range: 3.9 - 250 pg/ml Sensitivity: < 2.34 pg/ml
Please refer to the Manual