Brilliant Sodium Flex - No wash sodium assay kit

Catalogue Number: 9000-10-INB

Manufacturer:	Ion Biosciences
Type:	Detection Kit
Shipping Condition:	RT
Unit(s):	10 plates

Overview
Datasheets

Description

Description: No wash sodium assay, 10 plates. Total assay solution for plate-based, high-throughput measurements of intracellular sodium flux. Also endpoint assay compatible. Excitation: 525 nm | Emission: 545 nm ION's Brilliant Sodium Assay is a total assay solution for multi-well plate-based, high-throughput measurements of changes in intracellular Na⁺ mediated through a wide-variety of plasma membrane and intracellular sodium channels and transporters. In multi-well, plate-based formats, the Brilliant Sodium Assay can be used to discover and characterize the effects of many tens-of-thousands of compounds and environmental factors on effectors of intracellular Na⁺. ION's Brilliant Sodium Assay provides all the reagents necessary for use as a wash or no-wash assay with adherent or non-adherent cells. The optional use of a probenecid solution and an extracellular background masking solution (TRS) offers the ultimate in compatibility for cells types which are difficult to load with fluorescent Na⁺ indicators (e.g. Chinese Hamster Ovary, CHO cells) and when performing assays in complete, serum-containing cell culture medium is desired. ION's Brilliant Sodium Assay is compatible with fluorescence microscopes, flow cytometers, and plate readers capable of detecting fluorescein or more optimally, yellow fluorescent protein (YFP).

9000-10 Protocol