Cy5 NHS ester (non-sulfonated)

Catalogue Number: A8102-APE

Manufacturer:	Apexbio
Shelf Life:	24 months
Molecular Formula:	C36H42ClN3O4
Physical state:	A solid
Type:	Fluorescent Reagents
Shipping Condition:	Blue Ice
Unit(s):	10 mM (in 1mL DMSO), 5 mg

Overview
Datasheets

Description

Description: Cyanine5 NHS ester is a ideal reactive dye for the labeling of peptides, proteins, and oligonucleotides which contain the amino-groups. Recently, among the life science research and diagnostics, Dye color quantity can be detected in gel electrophoresis by naked eye as the dye color is very intense and as small as 1 nanomol. For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer. For more sophisticated targets such as easily degradable proteins, when use of DMF or DMSO is undesirable, consider using water-soluble Cy5 NHS ester which does not require co-solvent, and has very similar fluorescent properties.In mice, Cy5-labeled polyplexes was intravenous injected to evaluate the targeting ability of polyplexes in tumor-bearing nude mice. Heart, liver, spleen, lung, kidney and tumor, were removed after the mice were sacrificed and the fluorescence intensities of Cy5-labeled polyplexes were immediately examined [1].Reference:[1] Chen, K. ; Chen, Q.; Wang, K.; Zhu, J.; Li, W.; Li, W.; Qiu, L.; Guan, G.; Qiao, M.; Zhao, X.; Hu, H.; Chen, D. Synthesis and characterization of a PAMAM-OH derivative containing an acid-labile β-thiopropionate bond for gene delivery. International Journal of Pharmaceutics, 2016, 509(1-2), 314-327.

Additional Text

Short Description

Labeling of amino-groups in biomolecules.

Reagent:Smile String

CC1(C2=CC=CC=C2[N+](=C1C=CC=CC=C3C(C4=CC=CC=C4N3CCCCCC(=O)ON5C(=O)CCC5=O)(C)C)C)C

Solubility

≥30.8 mg/mL in DMSO; insoluble in H2O; ≥15.1 mg/mL in EtOH with ultrasonic

Storage Note

24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

A8102 Datasheet

A8102 CofA

A8102 MSDS

Price

£328.00

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