Cy3 NHS ester

Catalogue Number: A8107-APE

Manufacturer:Apexbio
Shelf Life:24 months
Molecular Formula:C35H41N3O10S2
Type:Fluorescent Reagents
Shipping Condition:Blue Ice
Unit(s): 25 mg, 5 mg

Description

Description: Sulfo-Cy3 NHS ester is a sulfonated, hydrophilic and highly water soluble dye. For labeling proteins and peptides, no organic co-solvent is needed. Sulfo-Cy3 NHS ester is ideal for proteins with low solubility and proteins prone to denaturation. Non-sulfonated Cy3 NHS ester is also available. In life science, the sulfonate groups make the Cy dyes soluble in water and also reduce fluorescence-quenching which can arise from dye-dye interactions. In vitro, sulfo-Cy3 NHS ester can react with green-emitting QDs ligand-exchanged and LA/His-PIMA-PEG-NH2 to provide QD-dye conjugates [1].Reference:[1] Reid, D.A.; Keegan, S.; Leo-Macias, A.; Watanabe, G.; Strande, N.T.; Chang, H.H.; Oksuz, B.A.; Fenyo, D.; Lieber, M.R.; Ramsden, D.A.; Rothenberg, E. Organization and dynamics of the nonhomologous end-joining machinery during DNA double-strand break repair. Proceedings of the National Academy of Sciences of the U.S.A., 2015, 112(20).

Additional Text

Short Description

Labeling of amino-groups in biomolecules.

Solubility

insoluble in EtOH; insoluble in DMSO; insoluble in H2O

Storage Note

24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Reagent:Smile String

O=C(ON1C(CCC1=O)=O)CCCCCN(C(C=CC(S(=O)(O)=O)=C2)=C2C/3(C)C)C3=CC=CC4=[N+](CC)C(C=CC(S(=O)([O-])=O)=C5)=C5C4(C)CC)C)C)C