Cy5 NHS ester
Catalogue Number: A8108-APE
| Manufacturer: | Apexbio |
| Shelf Life: | 24 months |
| Molecular Formula: | C37H43N3O10S2 |
| Type: | Fluorescent Reagents |
| Alias: | CY5-SE; Sulfo-Cyanine5 Succinimidyl Ester |
| Shipping Condition: | Blue Ice |
| Unit(s): | 25 mg, 5 mg |
Description
Description: Sulfo-Cy5 NHS ester is a sulfonated, hydrophilic and highly water soluble dye. For the labeling of various amine-containing molecules in aqueous phase, no organic co-solvent is needed. This regent is especially useful for the labeling of proteins which denaturate in the presence of organic co-solvents, as well as for proteins with low solubility. Sulfo-Cy5 NHS ester is one of the most popular fluorophores. In life science, the sulfonate groups make the Cy dyes soluble in water and also reduce fluorescence-quenching which can arise from dye-dye interactions.Sulfo-Cy5 NHS ester, was conjugated to LLP2A with 646/662 peak excitation/emission in dimethyl sulfoxide. LLP2A exhibited punctate staining around the cell periphery, consistent with it labeling VLA-4 [1].Reference:[1] Soodgupta, D. ; Zhou, H.; Beaino, W.; Lu, L.; Rettig, M.; Snee, M.; Skeath, J.; DiPersio, J.F.; Akers, W.J.; Laforest, R.; Anderson, C.J.; Tomasson, M.H.; Shokeen, M. Ex Vivo and In Vivo Evaluation of Overexpressed VLA-4 in Multiple Myeloma Using LLP2A Imaging Agents. Journal of Nuclear Medicine, 2016, 57(4), 640-645.
Additional Text
Short Description
Labeling of amino-groups in biomolecules.
Solubility
insoluble in H2O; insoluble in EtOH; insoluble in DMSO
Storage Note
24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
Reagent:Smile String
CC[N+]1=C(/C=C/C=C/C=C2N(CCCCCC(ON3C(CCC3=O)=O)=O)C(C=CC(S(O)(=O)=O)=C4)=C4C/2(C)C)C(C)(C)C5=C1C=CC(S([O-])(=O)=O)=C5
A8108 Datasheet
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