Catalogue Number: A8127-APE
Manufacturer: | Apexbio |
Shelf Life: | 24 months |
Molecular Formula: | C34H45N6NaO8S2 |
Type: | Click Chemistry Reagent |
Shipping Condition: | Blue Ice |
Unit(s): | 25 mg, 5 mg |
Description: Sulfo-Cy3 azide is a sulfonated, hydrophilic and highly water soluble dye for Click Chemistry. For labeling of various molecules in aqueous phase for brightness and photostability of fluorophore, sulfo-Cy3 azide is an ideal choice. Click reaction can be carried out in pure water without need to use organic co-solvent. For labeling proteins and even intact biological objects, no organic co-solvent is needed. In life science, the sulfonate groups make the Cy dyes soluble in water and also reduce fluorescence-quenching which can arise from dye-dye interactions. In vitro, sulfo-Cy3 azide was used for fluorescent microscopy staining. Human U87MG glioblastoma cells that overexpress uPAR were just stained by Cy3-AE105 conjugate [1].Reference:[1] Sun, L. Gai, Y. Anderson, C.J.; Zeng, D. Highly-efficient and versatile fluorous-tagged Cu (I)-catalyzed azide-alkyne cycloaddition ligand for preparing bioconjugates. Chemical Communications, 2015, 51, 17072-17075.
used for Click chemistry to label alkyne-modified oligonucleotides
CC1(C2=C(C=CC(=C2)S(=O)(=O)[O-])[N+](=C1C=CC=C3C(C4=C(N3CCCCCC(=O)NCCCN=[N+]=[N-])C=CC(=C4)S(=O)(=O)[O-])(C)C)C)C
≥10 mg/mL in DMSO; ≥16.67 mg/mL in EtOH; ≥16.67 mg/mL in H2O
24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.