Cy5 alkyne (non-sulfonated)

Catalogue Number: A8131-APE

Manufacturer:	Apexbio
Molecular Formula:	C35H42ClN3O
Physical state:	A solid
Type:	Fluorescent Reagents
Shipping Condition:	Blue Ice
Unit(s):	25 mg, 5 mg

Overview
Datasheets

Description

Description: Cy5 alkyne, with low aqueous solubility is a dye ready for the use in Click Chemistry reaction. Various molecules via Click Chemistry reaction can be attached by this deeply colored, and photostable Cy5 fluorophore. Most derivatives of non-sulfonated cyanines have low aqueous solubility except for hydrochlorides of hydrazides and amines. For biomolecule labeling, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer. Various substrates such as biomolecules, polymers, and solid surfaces which bearing azides can be used for the labeling. In the azido-GalNAc samples, alkyne-Cy3 and alkyne-Cy5 specifically label azido-proteins. Furthermore, researches also indicate that the Cy5 and Cy3 probe-protein conjugates have non-overlapping fluorescence profiles and can be differentially detected within a single gel [1].Reference:[1] Burnham-Marusich, A.R.; Plechaty, A.M.; Berninsone, P.M. Size-matched alkyne-conjugated cyanine fluorophores to identify differences in protein glycosylation. Electrophoresis, 2014, 35(18), 2621-2625.

Additional Text

Storage Note

Store at -20°C

Short Description

used for Click chemistry to label any molecule bearing azide group.

Reagent:Smile String

CC(C1=CC=CC=C1N/2C)(C)C2=C/C=C/C=C/C3=[N+](CCCCCC(NCC#C)=O)C(C=CC=C4)=C4C3(C)C.[Cl-]

Solubility

≥55.6 mg/mL in DMSO; insoluble in H2O; ≥50 mg/mL in EtOH with ultrasonic

Price

£1,080.00

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