Cy3 carboxylic acid (non-sulfonated)

Catalogue Number: A8132-APE

Manufacturer:Apexbio
Shelf Life:24 months
Molecular Formula:C30H37ClN2O2
Physical state:A solid
Type:Fluorescent Reagents
Shipping Condition:Blue Ice
Unit(s): 25 mg, 5 mg

Description

Description: Cy3 carboxylic acid is a non-activated fluorescence dye. Most derivatives of non-sulfonated cyanines have low aqueous solubility except for hydrochlorides of hydrazides and amines. This Non-sulfonated reagent has limited aqueous solubility, but with good solubility in organic solvents. For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer. In the supramolecular glutamine assay, Cy3 carboxylic acid was conjugated to a single strain DNA and then immobilized onto the glass slide through DNA hybridization. The Cy3 groups serve as FRET donor and the cyclodextrins as supramolecular host [1]. Reference:[1] Xue, M.; Wei, W.; Su, Y.; Johnson, D.; Heath, J.R. Supramolecular Probes for Assessing Glutamine Uptake Enable Semi-Quantitative Metabolic Models in Single Cells. Journal of the American Chemical Society, 2016, 138(9), 3085-3093.

Additional Text

Short Description

Non-reactive fluorophore, for control experiments, and for calibration

Reagent:Smile String

CC(C1=C(N/2C)C=CC=C1)(C)C2=C/C=C/C3=[N+](CCCCCC(O)=O)C(C=CC=C4)=C4C3(C)C.[Cl-]

Solubility

≥49.3 mg/mL in DMSO; insoluble in H2O; ≥99 mg/mL in EtOH

Storage Note

24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.