Cy3 Bis NHS ester
Catalogue Number: A8772-APE
| Manufacturer: | Apexbio |
| Molecular Formula: | C43H49N4NaO14S2 |
| Type: | Fluorescent Reagents |
| Shipping Condition: | Dry Ice |
| Unit(s): | 25 mg, 5 mg |
Description
Description: Cy3 Bis NHS ester is a reactive fluorescent dye for general labeling of protein amine groups. Cy3 Bis NHS ester includes two reactive groups on one dye molecule. The N-hydroxysuccinimde (NHS) ester is the most popular amine reactive group, used for capturing the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. Appling NHS chemistry allows one to conjugate a variety of fluorescent probes (e.g. Cy3, a traditional orange-fluorescent label which is highly water soluble) to primary amines. Thus, target biological compounds can be visualized by Cy3 fluorescence (~550 nm excitation, ~570 nm emission) monitored by various fluorometers, imagers, and microscopes. References:1. Nanda JS, Lorsch JR. Labeling a protein with fluorophores using NHS ester derivitization. Methods in Enzymology, 2014, 536: 87-94.2. Dyla M, Andersen JL, Kjaergaard M, et al. Engineering a Prototypic P-type ATPase Listeria monocytogenes Ca2+-ATPase 1 for Single-Molecule FRET Studies. Bioconjugate Chemistry, 2016, 27(9): 2176-2187.
Additional Text
Storage Note
Store at -20°C
Reagent:Smile String
CC(C1=CC(S(=O)([O-])=[O-])=CC=C1N/2CCCCCC(ON3C(CCC3=O)=O)=O)(C)C2=C/C=C/C4=[N+](CCCCCC(ON5C(CCC5=O)=O)=O)C(C=CC(S(=O)([O-])=[O-])=C6)=C6C4(C)C.[Na+]
Solubility
insoluble in EtOH; ≥41 mg/mL in DMSO; ≥54.6 mg/mL in H2O
Short Description
A reactive fluorescent dye for general labeling of protein amine groups
A8772 CofA
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