DIG 3'-End Oligonucleotide Labeling Kit with Digoxigenin-11-ddUTP

Catalogue Number: APP-002-JEN

Manufacturer:	Jena Bioscience
Shelf Life:	12 months
Type:	Oligonucleotides
Shipping Condition:	Blue Ice
Unit(s):	25 reactions x 50 ul

Overview
Datasheets

Description

Description: DIG 3'-End Oligonucleotide Labeling Kit with Digoxigenin-11-ddUTP contains all reagents (except oligonucleotide template to be labeled and materials for Digoxigenin detection) required for efficient 3'-End digoxigenylation of DNA oligonucleotides (length: 20 -100 bp, 5 pmol per reaction). The labeling principle is based on Terminal deoxynucleotidyl Transferase (TdT) that template-independently transfers 1 Digoxigenin-11-ddUTP to the 3'-OH group of ssDNA (e.g. an oligonucleotide) in the presence of CoCl2. It is similar to the principle of DIG Oligonucleotide 3'-End DNA Labeling Kit, 2nd Generation (Roche). The resulting 3'-End digoxigenylated oligonucleotides are ideally suited for applications involving sequence-specific protein binding or hybridiziation such as EMSA, Northern or Southern blots. Compared to internal, random digoxigenylated probes, Digoxigenin is located at the 3'-End only and less likely interferes with probe binding. TdT possesses a preference for single-stranded DNA (ssDNA) over dsDNA with 3'-overhangs or blunt ends. For the preparation of labeled dsDNA complexes, label each complementary oligonucleotide separately and anneal them before use.

APP-002 Datasheet

Price

£327.00

-

Quantity
Unit Size

Find out about delivery