EZ Cap™ Reagent AG
Catalogue Number: B8176-APE
| Manufacturer: | Apexbio |
| Molecular Weight: | 1145.60 (free acid) |
| Molecular Formula: | C32H43N15O24P4 (free acid) |
| Physical state: | Solution |
| Type: | Labelled Nucleotides |
| Alias: | EZ Cap™ Reagent AG for co-transcriptional capping of mRNA,m7G(5')ppp(5')(2'OMeA)pG |
| Shipping Condition: | Dry Ice |
| Storage Condition: | -20°C/-70°C |
| Unit(s): | 10 ul (100 mM), 100 ul (100 mM), 50 ul (100 mM) |
Description
Description: EZ Cap Reagent AG is a co-transcription capping reagent for in vitro transcription of 5'capped mRNA to produce Cap 1 structure. Cap AG has been shown in the literature to provide a capping efficiency of up to 98%.EZ Cap AG requires an AG initiator. EZ Cap AG typically provides >90% capped material generating a naturally occurring Cap 1 structure.EZ Cap is compatible with wild-type T7 polymerase. 5 mM EZ Cap and rNTPs is recommended.Template design is an integral part of any transcription. EZ Cap reagent AG can be used with a specific initiating sequence 5' AG 3'.Final capping efficiency depends on the EZ Cap Reagent, DNA template and final mRNA sequence. Secondary structure due to RNA length and base composition can affect final capping efficiency.
Additional Text
Short Description
Used for co-transcriptional capping of mRNA to form Cap 1 structure, m7G(5')ppp(5')(2'OMeA)pG.
Storage Note
Store at -20°C or below
Molecular Formula
C32H43N15O24P4 (free acid)
Molecular Weight
1145.60 (free acid)
Reagent:Smile String
O=C1NC(N)=NC2=C1[N+](C)=CN2[C@@H]3[C@@H](O)[C@@H](O)[C@H](COP([O-])(OP([O-])(OP([O-])(OC[C@H]4O[C@@H](N5C=NC6=C5N=CN=C6N)[C@H](OC)[C@@H]4OP([O-])(OC[C@H]7O[C@@H](N8C=NC9=C8N=C(N)NC9=O)[C@H](O)[C@@H]7O)=O)=O)=O)=O)O3.[Na+].[Na+].[Na+]
B8176 CofA
SUPPORT
outstanding technical support
PRODUCT
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DELIVERY
we offer free delivery to UK universities and non profit organisations