Catalogue Number: BMU101-EN-ABK
Manufacturer: | Abbkine |
Shelf Life: | 12 months |
Type: | Enzyme Substrates |
Alias: | Chemiluminescence|ECL|Electrochemiluminescence|Enhanced Chemiluminescence |
Shipping Condition: | Blue Ice |
Unit(s): | 100 ml, 500 ml |
Application: | IP, WB |
Description: The family of ECL substrates is a series of premixed, ready-to-use chemiluminescent reagents for the detection of HRP-based Westerns. The SuperKine™ West Pico PLUS Chemiluminescent Substrate is specially optimized for picogram amounts of antigen detection on immunoblots, with pouring directly onto the blot membrane without worrying about pipetting error. The substrate's intense signal output and duration enables detection of picogram amounts of antigen with photographic or other imaging methods perfectly well.
ECL
• Mix the two substrate components at a 1:1 ratio to prepare the substrate working solution. • Suggest 0.1-0.2ml of SuperLumia ECL working solution for 1 cm2 size of blot membrane. • Incubate blot 1-5 minutes in SuperLumia ECL Substrate working solution. • Drain excess reagent. Cover blot with clear plastic wrap. • Expose blot to X-ray film.