TGF beta 1 Polyclonal Antibody
Catalogue Number: BS-0086R-BSS
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Formalin-fixed and paraffin embedded rat renal tissue labeled with Anti-TGF-beta-1 Polyclonal Antibody, Unconjugated(bs-0086R) 1:200 followed by conjugation to the secondary antibody and DAB staining
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Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with TGF beta 1 Polyclonal Antibody, Unconjugated (bs-0086R) at 1:500 overnight at 4°C, DAB staining.
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Lane 1: Isolated Transforming growth factor beta-1; Probed with TGF beta 1 Polyclonal Antibody, Unconjugated (bs-0086R) at 1:300 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37°C.
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Lane 1: Mouse Embryo; Lane 2: Rat Cerebrum; Probed with TGF beta 1 Polyclonal Antibody, Unconjugated (bs-0086R) at 1:1000 dilution, overnight at 4°C. Followed by secondary antibody incubation at 1:10000 for 60 min at 37°C.
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Lane 1: Mouse Ovary; Probed with TGF beta 1 Polyclonal Antibody, Unconjugated (bs-0086R) at 1:1000 dilution, overnight at 4°C. Followed by secondary antibody incubation at 1:10000 for 60 min at 37°C.
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Lane 1: Mouse Liver lysates; Lane 2: Rat Liver lysates probed with TGF beta 1 Polyclonal Antibody, Unconjugated (bs-0086R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
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Lane 1: Mouse Stomach lysates; Lane 2: Mouse Liver lysates; Lane 3: Mouse Placenta lysates; Lane 4: Mouse NIH/3T3 cell lysates; Lane 5: Rat Liver lysates probed with TGF beta 1 Polyclonal Antibody, Unconjugated (bs-0086R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
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MG63 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1%PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with TGF beta 1 Polyclonal Antibody(bs-0086R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | CED; DPD1; TGFB; TGFbeta; Transforming growth factor beta-1; TGF-beta-1; TGFB1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human TGF-Beta 1 |
| Application: | ELISA, FC, ICC, IF, IHC-P, WB, IHC-Fr |
Description
Description: Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
Additional Text
Gene ID
7040
Gene Name
TGFB1
Uniprot ID
P01137
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Modification
Unmodified
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bs-0086R Datasheet
SUPPORT
outstanding technical support
PRODUCT
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DELIVERY
we offer free delivery to UK universities and non profit organisations