HSP60 Polyclonal Antibody
Catalogue Number: BS-0191R-BSS
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Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti CYP5A1/Thromboxane synthase Polyclonal Antibody, Unconjugated (bs-4019R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
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Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti HSP60 Polyclonal Antibody, Unconjugated (bs-0191R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
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Mouse liver lysates probed with Rabbit Anti-HSP60 Polyclonal Antibody (bs-0191R) at 1:300 overnight at 4°C. Followed by a conjugated secondary antibody (bs-0295G-HRP ) at 1:5000 for 90 min at 37°C.
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Mouse kidney lysates probed with Rabbit Anti-HSP60 Polyclonal Antibody (bs-0191R) at 1:300 overnight at 4°C. Followed by a conjugated secondary antibody (bs-0295G-HRP ) at 1:5000 for 90 min at 37°C.
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Mouse heart lysates probed with Rabbit Anti-HSP60 Polyclonal Antibody (bs-0191R) at 1:300 overnight at 4°C. Followed by a conjugated secondary antibody (bs-0295G-HRP ) at 1:5000 for 90 min at 37°C.
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Paraformaldehyde-fixed, paraffin embedded rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HSP60 Polyclonal Antibody, Unconjugated (bs-0191R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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Lane 1: Mouse Liver lysates; Lane 2: Mouse Adrenal gland lysates; Lane 3: Mouse NIH/3T3 cell lysates; Lane 4: Rat Adrenal gland lysates; Lane 5: Human Jurkat cell lysates; Lane 6: Human MDA-MB-231 cell lysates; Lane 7: Human HepG2 cell lysates; Lane 8: Human Hela cell lysates; Lane 9: Human K562 cell lysates probed with HSP60 Polyclonal Antibody, Unconjugated (bs-0191R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
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HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (HSP60) polyclonal Antibody, Unconjugated (bs-0191R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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MCF-7 Lysates probed with Rabbit Anti-HSP60 Polyclonal Antibody (bs-0191R) at 1:300 overnight at 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37°C.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | HLD4; CPN6; GROEL; HSP6; HSP65; SPG13; HSP-6; HuCHA6; 6 kDa heat shock protein, mitochondrial; 6 kDa chaperonin; Chaperonin 6; Heat shock protein 6; Mitochondrial matrix protein P1; P6 lymphocyte protein; HSPD1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human HSP60 |
| Application: | ELISA, ICC, IF, IHC-P, WB, IHC-Fr |
Description
Description: Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.
Additional Text
Gene Name
HSPD1
Gene ID
3329
Uniprot ID
P10809
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Modification
Unmodified
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bs-0191R Datasheet
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DELIVERY
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