FGF2 Polyclonal Antibody
Catalogue Number: BS-0217R-BSS
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Image provided by One World Lab validation program. A549 cells probed with Rabbit Anti-bFGF Polyclonal Antibody (bs-0217R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
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HepG2 lysates probed with bFGF Polyclonal Antibody, Unconjugated (bs-0217R) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37°C.
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Mouse spleen lysates probed with bFGF Polyclonal Antibody, Unconjugated (bs-0217R) at 1:300 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37°C.
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Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-bFGF Polyclonal Antibody, Unconjugated (bs-0217R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
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Formalin-fixed and paraffin embedded mouse kidney labeled with Anti-BFGF/FGF2 Polyclonal Antibody, Unconjugated (bs-0217R) followed by conjugation to the secondary antibody and DAB staining
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Molt-4 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20°C, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with bFGF Antibody(bs-0127R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | Fgfb; bFGF; Fgf-2; Fibroblast growth factor 2; Basic fibroblast growth factor; Heparin-binding growth factor 2; HBGF-2; Fgf2 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from mouse FGF2 |
| Application: | ELISA, FC, ICC, IF, IHC-P, WB, IHC-Fr |
Description
Description: Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro (By similarity).
Additional Text
Uniprot ID
P09038
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Modification
Unmodified
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Gene ID
14173; 2247
Gene Name
Fgf2, FGF2
bs-0217R Datasheet
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DELIVERY
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