Cyclin E1 Polyclonal Antibody
Catalogue Number: BS-0573R-BSS
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Lane 1: Lovo cell lysates; Lane 2: Mouse Thymus lysates; Lane 3: U2os cell lysates; Lane 4: K562 cell lysates probed with Cyclin E Polyclonal Antibody, Unconjugated (bs-0573R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
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Lane 1: 293T cell lysates; Lane 2: 293T cell lysates probed with Cyclin E1 Polyclonal Antibody, Unconjugated (bs-0573R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
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MCF-7 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20°C, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Cyclin E Polyclonal Antibody(bs-0573R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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NIH/3T3 cells probed with Rabbit Anti-RAGE Polyclonal Antibody (bs-0177R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated Secondary Antibody.
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L1 rat brain, L2 rat lung lysates probed (bs-0573R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted and observed band size: 45kDa.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | Ccne; CYCLE; G1/S-specific cyclin-E1; Ccne1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from rat Cyclin E |
| Application: | ELISA, FC, ICC, IF, IHC-P, WB, IHC-Fr |
Description
Description: The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described. Two additional splice variants were reported but detailed nucleotide sequence information is not yet available. Transcript Variant: This variant (1) contains a different 5' end region, which includes an upstream in-frame translation start codon, when compared to variant 2. The encoded protein has a 15 aa longer N-terminus, as compared to isoform 2.
Additional Text
Gene Name
CCNE1
Uniprot ID
P39949
Gene ID
25729
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Modification
Unmodified
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bs-0573R Datasheet
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