CD34 Polyclonal Antibody
Catalogue Number: BS-0646R-BSS
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Human Raji cells probed with CD34 Polyclonal Antibody, Unconjugated (bs-0646R) (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).
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Mouse heart lysates probed with CD34 Polyclonal Antibody, unconjugated (bs-0646R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.
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Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CD34 Polyclonal Antibody, Unconjugated (bs-0646R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
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Formalin-fixed and paraffin embedded mouse heart tissue labeled Anti-CD34 Polyclonal Antibody, Unconjugated (bs-0646R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
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Formalin-fixed and paraffin embedded human gastric adenocarcinoma labeled Anti-CD34 Polyclonal Antibody, Unconjugated (bs-0646R) at 1:200, followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, AF488 conjugated(bs-0295G-AF488)used at 1:200 dilution for 40 minutes at 37°C
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Lane 1: Huvec cell lysates; Lane 2: Molt-4 cell lysates probed with CD34 Polyclonal Antibody, Unconjugated (bs-0646R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
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Mouse brain lysates probed with Anti-CD34 Polyclonal Antibody, Unconjugated (bs-0646R) at 1:3200 in 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37°C.
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Mouse lung lysate probed with Anti-CD34 Polyclonal Antibody, Unconjugated (bs-0646R) at 1:300 overnight at 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37°C.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | Hematopoietic progenitor cell antigen CD34; CD34 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human CD34 |
| Application: | ELISA, FC, ICC, IF, IHC-P, WB, IHC-Fr |
Description
Description: Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
Additional Text
Gene Name
CD34
Gene ID
947
Uniprot ID
P28906
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Modification
Unmodified
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bs-0646R Datasheet
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